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The mitochondrial coxII intron has been lost in two different lineages of dicots and altered in others
Author(s) -
Rabbi Mohammed F.,
Wilson Kenneth G.
Publication year - 1993
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1993.tb15355.x
Subject(s) - biology , apocynaceae , intron , catharanthus roseus , botany , phylogenetic tree , genetics , gene
The central part of the mitochondrial coxII gene was amplified from 38 different dicots and two monocots using polymerase chain reaction. In 30 of the 40 plants studied, the amplified coxll gene‐fragment contains an intron, ranging from 930 bp in Capsicum (pepper) in Solanaceae to 1,635 bp in Ampelamus albidans (climbing milkweed) in Asclepiadaceae. The composition of this intron varies as revealed by Southern hybridizations using oligonucleotide probes specific to the coxII intron‐regions in maize, wheat, and rice. In the Apocynaceae, Calharanthus roseus (Madagascar periwinkle) and Vinca minor ( common periwinkle) lack the coxII intron, while other members of the same family (various Mandevilla species, Nerium oleander and Apocynum cannabinum ) and members of the closely related Asclepiadaceae ( Asclepias incarnata, Ampelamus albidans and Asclepias tuberosa ) retain the intron. Analysis of these data suggest a selective loss of the coxII intron from a plant, ancestral to both Catharanthus and Vinca , after the divergence of the Asclepiadaceae and Apocynaceae. The remaining eight plants from the Brassicaceae, Cucurbitaceae, Fabaceae, and Onagraceae lacking the intron fall into a single group or clade using the phylogenetic tree proposed by Chase et al. ( Annals of the Missouri Botanical Garden : 80: 528–580, 1993) based on sequence of the chloroplast rbcL gene.