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CELL‐ AND TISSUE‐SPECIFIC EXPRESSION OF RICE HISTONE GENE TRANSCRIPTS DURING ANTHER AND POLLEN DEVELOPMENT IN HENBANE (HYOSCYAMUS NIGER)
Author(s) -
Raghavan V.,
Jiang Changjun,
Bimal Raageeva
Publication year - 1992
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1992.tb13654.x
Subject(s) - tapetum , biology , stamen , pollen , botany , in situ hybridization , gene expression , gene , primordium , microbiology and biotechnology , microspore , genetics
We have monitored by in situ hybridization the cell‐ and tissue‐specific expression of a rice histone H3 gene during anther and pollen development in henbane ( Hyoscyamus niger ). Autoradiographic silver grains generated by hybridization of 35 S‐labeled antisense probe were present in more or less the same density in all the cells of the anther primordium. During later development of the anther, there was a sequential accumulation of histone mRNA beginning in the epidermis on the outside and ending in the tapetum inside. These changes in transcript abundance in the sporophytic cells of the anther were also related to the stage of microsporogenesis. Transcript level increased during sporogenesis and reached a maximum in mature pollen grains collected from open flowers.