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GENETIC STABILITY OF ANTHER CALLUS LINES AND PROGENY PLANTS OF MAIZE
Author(s) -
Ting Y. C.,
Gu M. G.
Publication year - 1990
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1990.tb15181.x
Subject(s) - biology , callus , stamen , ploidy , meiosis , botany , inbred strain , chromosome , mitosis , genetics , pollen , gene
Cytological observations showed that mitotic variations among the four callus lines were toward different directions; two to haploidy, one to diploidy, and the fourth to polyploidy. These differences might be caused by varying genetic backgrounds. Under appropriate conditions, certain regenerable callus lines could be maintained by continued subcultures for an indefinite period of time. Microscopic examinations revealed that meiotic chromosome fusion in maize anther culture‐derived R 0 plants were again found in the R 1 and R 2 plants. This suggests that a genetic mechanism was involved in bringing about this characteristic fusion. This mechanism probably arose de novo during culturing since no fusion was found in the parental lines. Through a rigid selection for healthy and vigorous plants for two to three generations, genetically stable inbred lines originating from maize anther culture have been established.