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STIGMA OF SOLANUM TUBEROSUM CV SHEPODY: MORPHOLOGY, ULTRASTRUCTURE,AND SECRETION
Author(s) -
Mackenzie Cynthia J,
Yoo Bong Y.,
Seabrook Janet E. A.
Publication year - 1990
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1990.tb13609.x
Subject(s) - biology , ultrastructure , secretion , staining , stain , botany , pollen tube , pollen , biochemistry , pollination , genetics
The stigma of Solarium tuberosum L. cv Shepody has a bilobed papillate surface covered with a viscous secretion at anthesis. The secretion originates as osmiophilic droplets in the cytoplasm, accumulates in the intercellular space, and fills the base of the papillae, after lifting and rupturing the cuticular layer covering the stigma surface. Cytochemical evidence shows that the stigmatic secretion is lipidic in nature; it did not stain with the periodic acid‐Schiffs reaction for carbohydrate or Coomassie Brilliant Blue R‐250 for proteins, but did stain with Sudan black or Nile red, a fluorescence probe for lipids. Sodium dodecylsulfate‐polyacrylamide gel electrophoresis has revealed that the secretion contained three polypeptides that appeared to be 'indigenous' to the stigmatic secretion, and not contaminants. Comparative analyses of this and other proteins found in stigma secretions may provide clues to their possible roles in pollen grain adhesion and germination.