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EMBRYOLOGICAL STUDY OF HERMINIUM MONORCHIS (ORCHIDACEAE) USING CONFOCAL SCANNING LASER MICROSCOPY
Author(s) -
Fredrikson Margit
Publication year - 1990
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1990.tb13535.x
Subject(s) - biology , embryology , endosperm , embryo , confocal laser scanning microscopy , gametophyte , orchidaceae , megaspore , confocal microscopy , botany , anatomy , confocal , ovule , microbiology and biotechnology , optics , biophysics , pollen , physics
The embryology of Herminium monorchis (Orchidaceae) was studied using confocal scanning laser microscopy (CSLM), a new technique for embryological studies. This technique may contribute new information to plant embryology. Herminium monorchis has a monosporic embryo sac development. The mature embryo sac is 8‐nucleate. Two integuments, both 2‐layered, are formed, but only the inner takes part in formation of the micropyle. Double fertilization takes place. The primary endosperm nucleus does not divide, but remains alive at least at the 3‐celled stage of embryo development. The three antipodals do not show any sign of degeneration at this stage.