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ADVENTIVE ORGANOGENESIS FROM SOMATIC TISSUE CULTURES OF SOLANUM CAROLINENSE: ORIGIN AND DEVELOPMENT OF REGENERATED PLANTS
Author(s) -
Reynolds Thomas L.
Publication year - 1989
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1989.tb11353.x
Subject(s) - biology , primordium , organogenesis , callus , explant culture , meristem , somatic embryogenesis , botany , shoot , cell division , microbiology and biotechnology , somatic cell , tissue culture , cytokinin , embryo , embryogenesis , auxin , cell , biochemistry , in vitro , gene
The development of adventitious shoot formation from cultured somatic tissues of Solanum carolinense was studied using light microscopy. For purposes of comparison, callus initiation and proliferation were also followed. When stem segments of this plant were cultured on medium supplemented with 6 mg/l benzyladenine (BA), cell division was first observed after 48 hr in the external phloem and inner cortex of the segments. This division activity gave rise to meristematic zones which subsequently formed shoot primordia within 6 days of culture. While organogenic potential appears to be limited to specific regions, all tissues of the explant were capable of callus formation when cultured on medium containing 3 mg/l 2,4‐dichlorophenoxyacetic acid (2,4‐D). In conjunction with a previous study, it appears that unlike somatic embryogenesis in this species, organogenesis does not require an intermediate callus phase for differentiation to occur.