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IN OVULO EMBRYO DEVELOPMENT AND PLANT FORMATION FROM STENOSPERMIC GENOTYPES OF VITIS VINIFERA
Author(s) -
Emershad Richard L.,
Ramming David W.,
Serpe Marcelo D.
Publication year - 1989
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1989.tb11327.x
Subject(s) - biology , embryo , ovule , polyembryony , asparagine , embryo culture , botany , glutamine , genotype , embryo rescue , embryogenesis , horticulture , amino acid , genetics , interspecific hybridization , gene , hybrid
Embryo enlargement and plant formation via in ovulo embryo culture was studied in stenospermic grapes. Genotypes, culture media and their interactions significantly affected embryo enlargement, whereas culture dates did not. Genotype P60‐58, when compared to cv. Thompson Seedless, had the greatest number of enlarged embryos after 3 months in culture. P60‐58 ovules cultured on Cain's basal medium plus L‐serine or L‐glutamine and cv. Thompson Seedless ovules cultured on Cain's basal medium plus L‐cysteine or L‐asparagine produced the greatest number of enlarged embryos. Viable embryos and morphologically normal‐appearing plants were obtained. Polyembryony was observed in cultured zygotic embryos.