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HISTOCHEMICAL LOCALIZATION OF ALCOHOL DEHYDROGENASE IN DEVELOPING BEAN SEEDS
Author(s) -
Yeung Edward C.,
Blackman Susan J.
Publication year - 1987
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1987.tb12137.x
Subject(s) - biology , alcohol dehydrogenase , embryo , dehydrogenase , staining , sorbitol dehydrogenase , cotyledon , coat , parenchyma , botany , biochemistry , enzyme , microbiology and biotechnology , genetics , paleontology
Alcohol dehydrogenase activity can be readily localized within bean seeds using the tetrazolium method. In the seed coat, prior to the cotyledon stage of embryo development, staining was seen throughout, especially in the branched parenchyma cells and the integumentary tapetum. As the embryo entered the maturation stage, a gradual decrease in the staining intensity was observed throughout the seed coat. Embryos stained well at all stages of development. In the course of this investigation, a high activity of “nothing dehydrogenase” was observed. Since its activity could be inhibited by methylpyrazole, an ADH inhibitor, it is concluded that the nothing dehydrogenase activity is caused by alcohol dehydrogenase.