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ULTRASTRUCTURAL LOCALIZATION OF CELL SURFACE IMMUNOSPECIFICITY IN ANABAENA AZOLLAE USING INDIRECT FLUORESCENT ANTIBODY STAINING
Author(s) -
Rosen Barry H.,
Johnson Robert C.,
Fisher Robert W.,
Gates James E.
Publication year - 1987
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1987.tb08716.x
Subject(s) - biology , ultrastructure , fluorescein isothiocyanate , immunogold labelling , staining , fluorescence , antiserum , biophysics , electron microscope , transmission electron microscopy , fluorescence microscope , microbiology and biotechnology , fluorescein , negative stain , cyanobacteria , antibody , botany , bacteria , materials science , optics , physics , genetics , immunology , nanotechnology
Antisera prepared against whole cells were used to differentiate between freshly isolated and cultured cyanobacteria from the nitrogen‐fixing fern Azolla filiculoides using both light and transmission electron microscopy (TEM). TEM suggested that the differentiating antigens are evenly distributed on the cell wall outer membrane of the cyanobacteria. The fluorescein isothiocyanate (FITC)‐conjugated secondary antibody accumulation was sufficiently electron‐dense to be detected without the need for either gold or ferritin labelling. Relative amounts of fluorescence which occurred on specimens viewed with fluorescent light microscopy were comparable to the thickness of electron dense material on the surfaces of these cells. Thus, it is possible to use the same immunological procedure to prepare cells for both light and TEM study.