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IN VITRO CULTURE OF EAR SHOOTS OF ZEA MAYS AND THE EFFECT OF KINETIN ON SEX EXPRESSION
Author(s) -
Bommineni V. R.,
Greyson R. I.
Publication year - 1987
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1987.tb08692.x
Subject(s) - kinetin , primordium , biology , inflorescence , stamen , shoot , botany , zea mays , explant culture , horticulture , in vitro , agronomy , pollen , biochemistry , gene
Immature ear primordia of maize when cultured on a defined liquid medium grew and differentiated in response to two variables: 1) size of initial explants and 2) concentration of kinetin in the medium. Overall growth of primordia, less than 15 mm at explanting, reached an optimum fresh weight and ear length when kinetin was 10 –6 m. Ears less than 10 mm, in the presence of kinetin, produced many male spikelets with well‐developed stamens in both upper and lower flowers. Ears greater than 15 mm, at explanting, produced only female flowers regardless of the kinetin concentration. Different proportions of male and female and bisexual flowers were produced depending upon the initial size of inflorescences and the concentration of kinetin.