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CHLOROPLAST DNA VARIABILITY AMONG LINUM SPECIES
Author(s) -
Coates David,
Cullis Christopher A.
Publication year - 1987
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1987.tb08604.x
Subject(s) - biology , chloroplast dna , linum , genetics , chloroplast , phylogenetics , restriction fragment , botany , dna , evolutionary biology , gene
We have compared the chloroplast DNA maps of a selection of Linum species, using recombinant DNA probes derived from L. usitatissimum chloroplast DNA. The heterologous probes have allowed us to construct physical maps for seven other Linum species. The chloroplast DNAs from two other species, L. flavum and L. tenuifolium , were so divergent that restriction maps could not be derived by this method. Analysis of the differences between the chloroplast DNAs has produced a phylogeny separating the species into two groups. These groups are coincident with previous taxonomic groupings and consist of L. perenne ‐related and non‐related species. The major difference between the chloroplast DNAs of the two groups is a 13‐kilobase pair segment near an inverted repeat/large single copy region boundary, which is present in the perenne ‐group species and absent from the non‐ perenne group species. In addition, we have identified a mutational hotspot analogous to that found in Nicotiana species chloroplast DNAs (Tassopulu and Kung, 1984). Among the species examined, the amount of base pair substitutions approaches 14% for the sites examined.