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TISSUE CULTURE DERIVED CROSSING BARRIERS
Author(s) -
Hughes Karen Woodbury
Publication year - 1986
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1986.tb12044.x
Subject(s) - biology , tissue culture , ovule , botany , germination , pollen , cell culture , mutant , wild type , embryo , microbiology and biotechnology , genetics , in vitro , gene
Tobacco plants derived from herbicide (paraquat)‐resistant cell lines and from wild type (non‐resistant) cell lines were self‐pollinated and crossed with sexually‐derived wild type plants. Analysis of the seed germination frequencies from these crosses indicated that seed viability and thus the ability of tissue culture‐derived plants to intercross with sexually derived plants was reduced. Partial reduction was observed when tissue culture‐derived plants were the female parent. Significant reduction in ability to intercross was observed when tissue culture‐derived plants were the male parent. Self pollinations of tissue culture‐derived plants and crosses between tissue culture‐derived plants, both wild type and mutant, did not show the presence of a crossing barrier. The crossing barrier (reduction of seed viability) is due to either: 1) failure of a fertilized ovule to undergo embryonic development, or 2) failure of pollen to fertilize ovules. It is apparently not due to in viable pollen. A possible explanation of the crossing barrier is that genetic changes accumulate as a result of the tissue culture process which reduces the ability of tissue culture‐derived plants to cross with sexually‐derived plants. Since tissue culture‐derived plants are able to intercross with each other and produce viable seed, the genetic changes would be similar in all of our tissue culture‐derived lines, regardless of mutant or wild type origin.