Premium
ANALYSIS OF STARCH CONTENT IN CHLORELLA PYRENOIDOSA (CHLOROPHYTA) BY MORPHOLOGICAL AND QUANTITATIVE TECHNIQUES
Author(s) -
Rosen Barry H.,
Berliner Martha D.,
Petro Michael J.
Publication year - 1986
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1986.tb10878.x
Subject(s) - chlorella pyrenoidosa , starch , biology , chlorophyta , quantitative analysis (chemistry) , chlorella , volume (thermodynamics) , chloroplast , botany , food science , chromatography , algae , biochemistry , chemistry , physics , quantum mechanics , gene
Chlorella pyrenoidosa (Chick) was grown heterotrophically in batch culture on defined medium with glucose. Morphometric analysis of cells in the exponential growth phase showed that starch accounted for 57% of the volume of the chloroplast and 36% of the total cell volume. During the stationary growth phase, the amount of starch accounted for only 36% of the chloroplast volume and 13% of the total cell volume. This represented a 36% decrease in the amount of starch/cell between the exponential and stationary phase. Determination of starch as grams/cell using quantitative techniques on cell extracts showed a comparable decrease in the amount of starch during this same transition. Based on these results, morphometric techniques provided an accurate assay of starch and have the added advantage of visualization of cellular structures not available when quantitative techniques are used.