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ACID‐INDUCED FILAMENT ELONGATION IN IPOMOEA NIL (CONVOLVULACEAE)
Author(s) -
Koning Ross E.
Publication year - 1986
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1986.tb09707.x
Subject(s) - fusicoccin , gibberellin , elongation , protein filament , biology , biophysics , efflux , botany , ipomoea , biochemistry , atpase , materials science , enzyme , ultimate tensile strength , metallurgy
It has been known for some time that morning glory filaments elongate in response to increases in concentration of gibberellins (Murakami, 1973) and decreases in ethylene production (Koning and Raab, in press), but many other aspects of their growth have remained unstudied. In the present work, the possible role of gibberellin‐stimulated proton efflux in filament growth was examined. Although applied gibberellins stimulated extensive filament growth in vitro and the pH of the incubating medium became acidified during growth, gibberellin also induced growth in media buffered at alkaline pH values. Acidic buffers alone elicited only a very small amount of growth. Fusicoccin, a potent stimulator of proton efflux, initially stimulated the rate of filament growth but elicited only a small increment of growth. In fact, continued presence of fusicoccin poisoned sustained gibberellin‐induced growth. Vanadate ions, believed to inhibit proton efflux, had little effect upon gibberellin‐induced growth except at extremely high concentrations. Based upon these results, it appears that the acid‐induced component of growth stimulation by gibberellin is relatively minor in Ipomoea filaments. These results are quite different from those reported for filament elongation in Gaillardia (Koning, 1983a).