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GRAFT INCOMPATIBILITY BETWEEN PEAR AND QUINCE: THE INFLUENCE OF METABOLITES OF CYDONIA OBLONGA ON SUSPENSION CULTURES OF PYRUS COMMUNIS
Author(s) -
Moore Randy
Publication year - 1986
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1986.tb09673.x
Subject(s) - pear , pyrus communis , biology , rosaceae , botany , glycoside , horticulture , tissue culture , biochemistry , in vitro
The fresh weights of suspension cultures of pear ( Pyrus communis ) and quince ( Cydonia oblonga ) increased exponentially for 30 to 40 days after subculturing. Transferring pear cultures to media in which quince cultures had grown for 10 days resulted in a 70% inhibition of callus growth. Transferring quince cultures to media in which pear cultures had grown for 10 days resulted in less than a 20% inhibition of growth. Addition of the cyanogenic glycosides amygdalin and prunasin (as 50 ppm CN _ ) killed pear cultures, while growth of quince cultures was inhibited by only approximately 50%. Addition of 50 ppm CN‐ severely inhibited growth of both cultures. These results indicate that 1) suspension cultures of quince release factor(s) that significantly inhibit growth of pear cultures, 2) quince cultures are relatively unaffected by metabolites released by pear cultures, 3) the severe inhibition of pear growth by quince metabolites is mimicked by the addition of cyanogenic glycosides ubiquitous to vegetative portions of quince, 4) direct cellular contact is not necessary to elicit incompatibility between pear and quince, and 5) incompatibility between pear and quince need not be associated with any particular stage of graft development.