Premium
DIRECT EMBRYOGENESIS FROM CULTURED ANTHERS AND PISTILS OF DACTYLIS GLOMERATA
Author(s) -
Songstad D. D.,
Conger B. V.
Publication year - 1986
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1986.tb08542.x
Subject(s) - dactylis glomerata , biology , gynoecium , stamen , botany , callus , ovary , dicamba , somatic embryogenesis , embryogenesis , microbiology and biotechnology , pollen , embryo , poaceae , genetics , ecology , weed control
Direct somatic embryoids were initiated from orchardgrass ( Dactylis glomerata L.) anthers and unpollinated pistils cultured in the dark at 25 C on Schenk and Hildebrandt (SH) medium supplemented with 30 μM dicamba (3,6 dichloro‐o‐anisic acid). Stereoscopic and scanning electron microscopy indicated that the embryoids originated from anther walls and from ovary and style regions of pistils. Callus initiation from direct embryoids leading to secondary embryogenesis was observed in pistils cultured from 4–6 wk. The ability of these calli to proliferate and initiate new embryoids through the dedifferentiation and redifferentiation of preexisting embryoids suggests long‐term totipotency.