Premium
ESTABLISHMENT OF LONG‐TERM CALLUS CULTURES FROM MATURE WHITE PINE (PINUS STROBUS, PINACEAE)
Author(s) -
Kaul Karan
Publication year - 1986
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1986.tb08526.x
Subject(s) - callus , biology , shoot , botany , pinaceae , horticulture , pinus <genus>
Callus cultures were established from young vegetative shoots of 15 to 18‐year‐old white pine trees. The callus has been repeatedly subcultured for 15 months on a modified Murashige and Skoog's medium supplemented with 2 mg/L 6‐Benzyladenine and 0.2 mg/L α‐Naphthaleneacetic acid. Best callus proliferation occurred when 2 to 4‐week‐old shoots were used as expiants. Older shoots were heavily contaminated with microorganisms and could be only partially disinfected by heat or chemical treatments.