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CELL CYCLE DURATION IN THE MERISTEM OF NEPHROLEPIS BISERRATA STOLONS: THE ROLE OF THE APICAL CELL
Author(s) -
Seilhean V.,
MichauxFerriere N.
Publication year - 1985
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1985.tb08356.x
Subject(s) - meristem , biology , cell cycle , stolon , colchicine , apical cell , mitosis , cell , cell division , microbiology and biotechnology , metaphase , botany , cell growth , chromosome , biochemistry , genetics , shoot , gene
The stolons of Nephrolepis biserrata (sw.) Schott are thin axes that grow rapidly (from 2 to 4 mm per day) in the controlled conditions applied. In the cylindro‐conical meristem, three histological zones are defined. Cell cycle duration was determined for each zone by autoradiographic methods after incorporation of tritiated thymidine and confirmed by the colchicine‐induced metaphase‐accumulation technique. The apical cell and its derivatives (Zone 1) are mitotically more active (cell cycle duration: 80 hr) than the cells of the subapical zones (2 and 3), where cell cycle lengths are 142 hr and 95 hr respectively. These data, compared to previous results, give evidence for the main role played by the relative rate of division of the apical cell compared to that of lateral cells in the organization and the shape of the meristem of pteridophytes. Moreover, the apical cell appears to be unique in having a differentiated cytological aspect not usually associated with an intensely proliferating cell.