Premium
INFLUENCE OF OCTOPINE, CALCIUM AND COMPOUNDS THAT AFFECT CALCIUM TRANSPORT ON ZEATIN‐INDUCED BUD FORMATION BY PYLAISIELLA SELWYNII
Author(s) -
Spiess Luretta D.,
Lippincott Barbara B.,
Lippincott James A.
Publication year - 1984
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1984.tb11998.x
Subject(s) - calcium , octopine , zeatin , biology , biochemistry , botany , chemistry , cytokinin , auxin , agrobacterium tumefaciens , organic chemistry , transgene , gene
Decreasing the calcium chloride content of the medium for the moss, Pylaisiella selwynii (Kindb.) Steere and Anderson, reduces the number of developed buds obtained in the presence of zeatin (1 μ m ), whereas similar changes in magnesium sulfate concentration have no effect. Cobalt (10 μ m ), lanthanum (10 μ m ), zinc (10 μ m ) and cadmium (0.01 μ m ) chlorides inhibit zeatin‐induced bud formation even in the presence of 680 μ m calcium, the normal concentration in the medium. Chlorpromazine (0.1 μ m ), cyclic adenosine 3′,5′‐monophosphate (100 μ m ), and glutamate (100 μ m ) also inhibit zeatin‐induced bud initiation. Ethylene glycol‐bis(β3‐aminoethyl ether)N,N,N′,N′‐tetraacetic acid (1 μ m ), the antibiotic A23187 (100 μ m ) and octopine (100 μ m ) each act synergistically with zeatin in promoting bud development and are generally more effective at calcium concentrations of 6.8 μ m or less. The combination of either ethylene glycol‐bis(β‐aminoethyl ether)N,N,N′,N′‐tetraacetic acid, A23817 or octopine with zeatin plus any of the inhibitory metals or compounds results in more buds than are obtained with zeatin alone and these combinations are more effective relative to zeatin response at calcium concentrations lower than 6.8 μ m . Experiments with Funaria hygrometrica (L.) Sibth. indicate a similar response to calcium, octopine and glutamate. These results appear to be related through changes in calcium availability at or within moss protonemal cells.