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DEVELOPMENTAL POTENTIAL OF EXCISED PRIMORDIAL AND EXPANDING LEAVES OF COLEUS BLUMEI BENTH.
Author(s) -
Smith Roberta H.
Publication year - 1984
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1984.tb11964.x
Subject(s) - primordium , kinetin , biology , coleus , explant culture , auxin , shoot , botany , cytokinin , rosette (schizont appearance) , horticulture , in vitro , biochemistry , gene , immunology
Coleus blumei Benth. primordial leaves 1 through 4 and expanding leaves 5 to 8 were isolated and cultured to examine the effects of auxin and kinetin on development. Without the plant growth regulators in the medium, expanding leaves 7 and 8 developed as leaves; younger leaf primordia did not develop. With 0.01 to 5.0 mg/1 IAA, 2–7% of the youngest pair of primordial leaves (1 and 2) developed as roots. Small leaf blade development occurred on IAA at 0.5 to 5.0 mg/1 with 10–12% of the explants, and shoots developed from 2% of the youngest primordia explants at 3 mg/1 IAA. With 2–28% of the second set of primordial leaves (3 and 4), a leaf with a root developed with 0.01 to 5.0 mg/1 IAA. At 3.0 mg/1 IAA, in addition to leaf formation, 2% of the explants formed a rosette of leaves and 1% formed a shoot. With the highest level of IAA (5 mg/1), 2% of the explants formed a root. Expanding leaves 5 through 8 developed mostly into leaves without petioles on IAA and kinetin. Plant development occurred from 2% of the youngest primordial leaves on 0.03 mg/1 kinetin; otherwise, these primordia on 0.003 to 2 mg/1 kinetin developed into abnormal leaves. Primordia 3 and 4 developed into normal appearing leaves at levels of kinetin between 0.03 and 2 mg/1. At lower levels the leaves were abnormal.