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INHERITANCE OF NUCLEAR 2C DNA CONTENT VARIATION IN INTRASPECIFIC AND INTERSPECIFIC HYBRIDS OF MICROSERIS (ASTERACEAE)
Author(s) -
Price H. James,
Chambers Kenton L.,
Bachmann Konrad,
Riggs Jennifer
Publication year - 1983
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1983.tb12462.x
Subject(s) - biology , intraspecific competition , nuclear dna , interspecific competition , hybrid , ploidy , gc content , dna , botany , genetics , zoology , mitochondrial dna , gene , genome
Nuclear DNA content varies over 20% within the diploid (2 n = 18) species M. douglasii and M. bigelovii. Two different intraspecific crosses were made between M. douglasii biotypes which differed by about 10% in 2C nuclear DNA content. The F 2 progeny of one intraspecific cross showed no striking evidence of segregation for DNA content. The mean DNA contents of F 2 progeny from two sister hybrids from the second intraspecific cross were significantly different at the 1% level. An interspecific cross was made between biotypes of M. douglasii and M. bigelovii that differed by approximately 10% in DNA amount. The 12 F 1 progeny did not cluster around the parental midpoint, but instead encompassed nearly the entire range between the parental means. The five families of F 2 progeny studied each had a mean DNA content corresponding to that of the particular F 1 from which they were derived, indicating that the F 1 plants were not of identical DNA content. The results of this study suggest that DNA sequences which account for the DNA content differences among the plants are unstable and can undergo deletion or amplification in a hybrid. The altered DNA content may be heritably stable and show little or no segregation in the F 2 progeny.