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PRODUCTION OF ANOMALOUS STRUCTURES IN QUERCUS RUBRA L. CALLUS CULTURES
Author(s) -
Seckinger Gary R.,
McCown Brent H.,
Struckmeyer B. Esther
Publication year - 1979
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1979.tb06313.x
Subject(s) - biology , callus , botany , meristem , organoid , shoot , vascular tissue , stele , epidermis (zoology) , agar , microbiology and biotechnology , anatomy , genetics , bacteria
Internodal stem segments of 2–4‐month‐old Quercus rubra L . seedlings were cultured on Murashige and Skoog agar medium containing NAA and BA. Structures, termed organoids, were initiated from callus on medium containing 5 mg/1 NAA and 0.1 mg/1 BA. Organoids consisted of parenchymatous cells with a distinct epidermis and a continuous vascular system. Growth occurred principally by cell division throughout the parenchymatous tissue resulting in a variety of morphological shapes. Although no organized shoot meristems were observed, normal roots were produced; such roots were connected to the vascular system of the organoid.