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EMBRYOGENIC DETERMINATION AND RIBONUCLEIC ACID SYNTHESIS IN POLLEN GRAINS OF HYOSCYAMUS NIGER (HENBANE)
Author(s) -
Raghavan V.
Publication year - 1979
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1979.tb06190.x
Subject(s) - pollen , stamen , biology , botany , incubation , uridine , rna , biochemistry , gene
3 H‐uridine administered as a one‐ or two‐hour pulse to embryogenic pollen grains of freshly excised anthers of Hyoscyamus niger (henbane) was autoradiographically localized in embryoids formed during a subsequent chase. Although continuous incubation of anthers in actinomycin D inhibited embryogenesis, a small percentage of potentially embryogenic pollen escaped inhibition if anthers were grown for at least one hour in the basal medium before actinomycin treatment. The results imply that certain pollen grains become embryogenically determined immediately after culture of the anther and that this is accompanied by the synthesis of ribonucleic acid.