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ZINC AND COPPER TOLERANCE OF AGROSTIS STOLONIFERA L. IN TISSUE CULTURE
Author(s) -
Wu Lin,
Antonovics Janis
Publication year - 1978
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1978.tb06067.x
Subject(s) - agrostis stolonifera , biology , callus , tissue culture , agrostis , botany , meristem , shoot , zinc , clone (java method) , copper , poaceae , biochemistry , chemistry , dna , organic chemistry , in vitro
Callus tissue was induced from shoot meristematic tissue and root tips of a clone of the grass Agrostis stolonifera tolerant to both zinc and copper, and from a control clone tolerant to neither metal. Growth of the callus tissue on media containing zinc and copper showed that tolerance to both metals was maintained in tissue culture. The pattern of metal uptake in tissue culture resembled uptake by whole plants in that tolerant tissue took up more metal than nontolerant tissue. Plants regenerated from callus had the same copper and zinc tolerance as the original parental clones regardless of time of growth in tissue culture and shoot or root origin of the tissue. The results support previous evidence that metal tolerance is genetically determined and acts at the cellular level.