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THE LATERAL MERISTEM AND ITS DERIVATIVES IN THE CORM OF ISOETES MURICATA
Author(s) -
Kruatrachue Maleeya,
Evert Ray F.
Publication year - 1977
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1977.tb15732.x
Subject(s) - cambium , biology , meristem , vascular cambium , botany , sieve (category theory) , sieve tube element , ultrastructure , primordium , plastid , cell division , phloem , xylem , biochemistry , chloroplast , cell , mathematics , combinatorics , shoot , gene
Corm tissue of Isoetes muricata Dur. was fixed in glutaraldehyde and postfixed in osmium tetroxide for electron microscopy. Very young secondary sieve elements can be distinguished from contiguous cambial cells by their distinctive plastids and by the presence of crystalline and/or fibrillar proteinaceous material in dilated cisternae of rough endoplasmic reticulum (ER). At maturity, the sieve elements are lined by the plasmalemma and a parietal, anastomosing network of smooth ER. Degenerate nuclei persist in all mature sieve elements. In addition, mature sieve elments contain plastids and mitochondria. Sieve‐area pores are present in all walls. The lateral meristem of I. muricata consists of 2–3 layers of cells year‐round. Judging from numerous collections made between October 1972 and July 1975, new sieve‐element differentiation precedes cambial activity by about a month. Early in May, 1–2 cells immediately adjacent to already mature sieve elements differentiate directly into sieve elements without prior division. In early June, at about the time sieve‐element differentiation is completed, cambial division begins. Division is sporadic, not uniform throughout the meristem. Dormancy callose accumulates in the secondary sieve elements in late October, and is removed in early May, at about the same time new sieve‐element differentiation begins. Cells of the dormant cambium are characterized by the presence of numerous small vacuoles and large quantities of storage materials, including lipid droplets, starch grains, and tannin. By contrast, active cambial cells contain few large vacuoles with little or no tannin, and they have little storage material.