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FURTHER CYTOLOGICAL STUDIES OF A PERIODIC ACID‐SCHIFF'S SUBSTANCE IN THE OVULES OF PASPALUM ORBICULARE AND P. LONGIFOLIUM
Author(s) -
Chao Chuanying
Publication year - 1977
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1977.tb11937.x
Subject(s) - integument , integumentary system , ovule , biology , fibril , botany , biophysics , cell wall , anatomy , pollen
A periodic acid‐Schiff's substance present in the micropylar end of the ovules of Paspalum orbiculare and P. longifolium was further studied by light and electron microscopy of glutaraldehyde‐osmium‐fixed and freeze‐substituted, osmium‐fixed tissues. The PAS substance is water soluble and is found in intercellular spaces between the nucellus and inner integument, the inner and outer integuments, the outer integument and ovary wall, and in the micropyle. Structurally the substance consists of fibrils embedded in a dense, amorphous matrix and may be associated with membranous structures in special layers between the plasmalemma and the cell wall in nucellar and integumentary cells. Part of the water soluble substance is believed to be secreted from the nucellar and integumentary cells. A large amount of this substance may be formed as a result of the dissolution of about one third of the distal micopylar portion of the outer integument prior to anthesis. Many of the electron‐dense fibrils seem to be fibrillar intercellular substances and others appear to originate from the cell walls, including the cuticle. Both the matrix and the fibrils may be chemically heterogeneous and together form a mucilagenous substance which may facilitate the final growth of pollen tubes in these two species.