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THE REQUIREMENT OF PHYSICAL CONTACT FOR MOSS GAMETOPHORE INDUCTION BY AGROBACTERIUM TUMEFACIENS
Author(s) -
Spiess Luretta D.,
Lippincott Barbara B.,
Lippincott James A.
Publication year - 1976
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1976.tb11818.x
Subject(s) - agrobacterium tumefaciens , moss , agrobacterium , biology , bacteria , strain (injury) , microbiology and biotechnology , botany , biochemistry , transformation (genetics) , genetics , anatomy , gene
Gametophore induction in the moss Pylaisiella selwynii by Agrobacteriuin tumefaciens strain B6 does not occur in parabiotic chambers when the moss and bacterium are separated by filters of 0.22 μm pore diameter. The combination of moss plus bacteria which gives increased gametophore formation also had no effect on sterile moss plants separated from the mixture by these filters. Heat‐killed B6 cells inhibit gametophore induction by viable B6 cells when added prior to or with the viable bacteria but not when added 6 or more hr later. The concentration dependence of both gametophore induction by viable B6 cells and inhibition of this process by heat‐killed B6 cells appears linear, suggesting that each response may require only one particle. Gametophore induction by Agrobacterium , therefore, requires physical contact comparable to the bacteria‐site attachment step demonstrated essential for tumor induction in higher plants and shows similar concentration dependence. The size of the moss and the absence of a wound requirement for this response makes the Agrobacterium ‐moss system particularly advantageous for further resolution of the site attachment step in Agrobacterium tumorigenesis.