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HISTOLOGICAL COMPARISON OF SINGLE AND ADDITIVE O 3 AND SO 2 INJURIES TO ELONGATING PONDEROSA PINE NEEDLES
Author(s) -
Evans Lance S.,
Miller Paul R.
Publication year - 1975
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1975.tb14065.x
Subject(s) - parenchyma , biology , cytoplasm , organelle , duct (anatomy) , epidermis (zoology) , anatomy , botany , microbiology and biotechnology
Visual symptoms and histological effects of SO 2 , O 3 , and a combination of SO 2 and O 3 were compared in rapidly elongating needles of sensitive clonal selections of Pinus ponderosa Laws. Both pollutants were administered separately or in combination at 0.45 ppm for 9 hr/day. Visual and histological injury appeared in distal needle portions after exposure to SO 2 or SO 2 plus O 3 ; but damage was predominately due to SO 2 . Ozone caused an aggregation of cytoplasm and cellular organelles into peripheral areas of plicate parenchyma cells. Ozone injury, which became evident several days after SO 2 damage, prevailed mostly on needle sections 10–15 mm from the distal tip. Sulfur dioxide caused dissolution of cellular components in virtually all needle cell types (parenchyma, epidermis, hypodermis, resin duct epithelium, and vascular tissues). Injury from SO 2 and O 3 is compared with that produced by other abiotic agents in pine in order to outline histological techniques for diagnostic purposes.