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UPTAKE BY AND FATE OF LYSOZYME IN ROOTS OF IASIONE MONTANA (CAMPANULACEAE)
Author(s) -
Sung Z. R.,
McLaren A. D.
Publication year - 1974
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1974.tb12291.x
Subject(s) - lysozyme , biology , fluorescein isothiocyanate , fluorescein , fluorescence , campanulaceae , botany , chromosomal translocation , cell wall , biophysics , microbiology and biotechnology , biochemistry , physics , quantum mechanics , gene
A basic protein, lysozyme, labeled with fluorescein isothiocyanate, is readily taken up by roots of lasione montana. Most of the protein taken up is tightly bound to cell walls of the roots. Fluorescent protein is diluted in the growing region of a root as cells elongate and divide. Fluorescence remains in mature nongrowing regions and root cap cells for one to two weeks. Redistribution and translocation of the protein within the root is minimal or nil. A layer of chloroform‐soluble material that prevents lysozyme from interacting with stem cell walls exposed to fluorescent lysozyme was found on stems of germinating Iasione montana.