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CYTOPLASMIC INCOMPATIBILITY STUDIES IN THE MYXOMYCETE DIDYMIUM IRIDIS: RECOVERY AND NUCLEAR SURVIVAL IN HETEROKARYONS
Author(s) -
Ling Hubert,
Upadhyaya Kailash C.
Publication year - 1974
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1974.tb12281.x
Subject(s) - heterokaryon , biology , cytoplasm , somatic cell , genetics , phenotype , microbiology and biotechnology , gene , mutant
At least eleven somatic compatibility loci exist in the myxomycete Didymium iridis. Cell fusion is controlled by at least seven fusion loci (Fus1–Fus7). Cytoplasmic compatibility is controlled by at least four clear‐zone loci (Cz1–Cz4). Plasmodia with identical phenotypes at all seven fusion loci, but different phenotypes at the clear‐zone loci, will fuse temporarily, but fusion is soon blocked by cytoplasmic reactions which prevent complete mixing. Areas which contain cytoplasm from two incompatible Plasmodia become clearly delineated from healthy cytoplasm. Such areas, termed clear zones, have been isolated and found to recover. If clear zones are sectioned into several small pieces, not all pieces will recover, indicating that toxic cytoplasmic reactions have occurred. Plasmodial fusion studies and F 1 studies of recovered clear zones indicate that the clear‐zone loci may also control nuclear survival in heterokaryons.