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FORMATION AND DISSOCIATION OF CELL AGGREGATES IN SUSPENSION CULTURES OF PAUL'S SCARLET ROSE
Author(s) -
Wallner Stephen J.,
Nevins Donald J.
Publication year - 1973
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1973.tb10225.x
Subject(s) - kinetin , cell division , biology , callus , biophysics , cell , cell culture , botany , hydrolysate , tissue culture , microbiology and biotechnology , biochemistry , in vitro , genetics , hydrolysis
In liquid culture stem tissue of Paul's Scarlet rose produces a suspension containing cell aggregates of extremely variable dimensions. There is, however, a definite pattern of change in the degree of cell aggregation over time. During the period of most rapid cell division large aggregates form as the result of a minimal separation of the proliferating cells. As the rate of cell division slows, the average number of cells per aggregate decreases. The dissociation of cell aggregates continues at a uniform rate after cell division has stopped. Cell separation is inhibited at low (0.1 mg/1) auxin (NAA) concentrations and by substitution of sucrose for glucose in the culture medium. Cell separation is delayed (but not greatly inhibited) by kinetin. The presence of casein hydrolysate prevents the formation of the large cell aggregates normally occurring in the early stages of the culture cycle. A variant strain which shows a much higher degree of cell separation has been isolated from stock callus tissue grown on solid medium.