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THE EFFECTS OF PLANT GROWTH SUBSTANCES ON IN VITRO FIBER DEVELOPMENT FROM FERTILIZED COTTON OVULES
Author(s) -
Beasley C. A.,
Ting Irwin P.
Publication year - 1973
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1973.tb10209.x
Subject(s) - kinetin , gibberellic acid , abscisic acid , biology , ovule , anthesis , botany , fiber , stimulation , horticulture , tissue culture , biochemistry , in vitro , chemistry , germination , cultivar , pollen , organic chemistry , neuroscience , gene
Cotton ovules were aseptically removed from ovaries 48 hr after anthesis and floated on the surface of liquid medium. Plant growth substances were filter sterilized and added to the medium in which glucose was the principal source of carbohydrate and KNO 3 the sole source of nitrogen. The amount of total fiber produced from the ovule surface was determined colorimetrically from the intensity of a destaining solution to which the ovules had been transferred following their 15‐second emersion in a solution of toluidine blue O. Gibberellic acid induced a marked stimulation and kinetin and abscisic acid a marked inhibition of fiber production from fertilized cotton ovules. Indoleacetic acid afforded only a trend of stimulation of fiber production. Gibberellic acid overcame the inhibition of total fiber production induced by both kinetin and abscisic acid. Indoleacetic acid did not overcome the inhibition of total fiber production induced by kinetin but did, to a great extent, overcome the inhibition due to abscisic acid.