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STARCH GEL ELECTROPHORESIS OF ENZYMES FROM NINE SPECIES OF POLYPORUS
Author(s) -
Shan Michael C.,
Ballal S. K.,
Harris John W.
Publication year - 1973
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1973.tb10203.x
Subject(s) - polyporus , biology , esterase , acid phosphatase , agar , phosphoglucomutase , agar gel , catechol oxidase , peroxidase , starch , alkaline phosphatase , enzyme , fungus , amylase , botany , biochemistry , polyphenol oxidase , bacteria , microbiology and biotechnology , genetics
Taxonomic relationships among 22 isolates of nine species of the wood‐rotting fungus, Polyporus , were examined by starch gel electrophoresis, coupled with histochemical staining procedures. Crude extracts of 21‐day cultures grown on malt extract agar were subjected to electrophoresis for 3 hr at a constant current of 25 ma. Esterase, peroxidase, catechol oxidase, galactose dehydrogenase, acid phosphatase, and alkaline phosphatase banding patterns were analyzed. With each enzyme, the bands for each isolate were compared with those for other isolates of the same species and for isolates of other species. A closer relationship was found in the banding patterns for different isolates of a single species than among isolates of different species. This technique may prove valuable for the evaluation of taxonomic differences among these wood‐rotting fungi.