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SUCROSE‐INDOLE‐3‐ACETIC ACID INTERACTIONS ON ROOT REGENERATION BY PINUS LAMBERTIANA EMBRYO CUTTINGS
Author(s) -
Greenwood Michael S.,
Berlyn Graeme P.
Publication year - 1973
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1973.tb10194.x
Subject(s) - sucrose , cutting , biology , botany , indole 3 acetic acid , auxin , horticulture , biochemistry , gene
Interactions affecting root formation by IAA and sucrose, supplied to Pinus lambertiana embryo cuttings through their cotyledons, were explored. The sucrose optimum for rooting and dry weight increase is approximately 8 %. Osmotic substitutes for sucrose, applied alone or concomitantly with sucrose, were unable to duplicate its effects on regeneration. One μM IAA increased the number of roots per cutting with all sucrose concentrations. However, with suboptimal sucrose concentrations it only increased the total number of cuttings forming roots. Because IAA accumulates at the site of root formation, the possibility that it mobilizes sucrose or its derivatives to the site of root formation was explored. No evidence was found for hormone‐directed transport of sucrose in this system. IAA had no effect on either the basal accumulation or transport of label derived from U‐ 14 C‐sucrose.