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QUANTITATIVE MICROSPECTROPHOTOMETRY OF NUCLEAR DNA IN SELFING STRAINS OF THE MYXOMYCETE DIDYMIUM IRIDIS
Author(s) -
Yemma John J.,
Therrien C. Dale
Publication year - 1972
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1972.tb10157.x
Subject(s) - biology , ploidy , selfing , heterothallic , nuclear dna , genetics , dna , mating type , feulgen stain , meiosis , botany , gene , mitochondrial dna , population , demography , sociology
Genetic and cytochemical investigations of the origin, development, nuclear activity, and ploidy level of Plasmodia obtained from selfed clones S‐2 and B 1 P‐33 of the heterothallic myxomycete, Didymium iridis , are presented. To demonstrate that selfing did not result from contamination of the clones, or mutations at the mating‐type locus, crosses were made between F 1 clones and clones of known mating types. The data were inconsistent with these two possibilities. DNA was quantified by Feulgen‐DNA microspectrophotometry. All cellular phases studied (logarithmic amoebae, swarmers, and encysted amoebae) appear to be haploid, with the nuclear DNA being in the replicated (2C) state. The plasmodia are in all cases diploid; however, the data indicate that the selfed Plasmodia are in an extended G 1 condition. The nuclear DNA content of these is therefore 2C, whereas that of the cross Plasmodium is 4C. Sporangial nuclei exhibit DNA in diploid replicated (4C) category.