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CARBOHYDRATE PHYSIOLOGY OF ORCHID SEEDLINGS. II. HYDROLYSIS AND EFFECTS OF OLIGOSACCHARIDES
Author(s) -
Ernst Robert,
Arditti Joseph,
Healey Patrick L.
Publication year - 1971
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1971.tb10036.x
Subject(s) - biology , hydrolysis , galactose , galactosides , biochemistry , invertase , sugar , extracellular , carbohydrate , botany , sucrose
Orchid seedlings were grown on nutrients containing cold‐sterilized sugars as sources of carbon. Action by an extra‐cellular invertase was implied from the hydrolysis by seedlings of sugars containing α‐D‐glucopyranosyl‐(1 → 2)‐β‐D‐fructofuranoside bonds. D‐galactosides (α and β) were also hydrolyzed, indicating the presence of extracellular galactosidases. Hydrolysis of α‐D‐fructofuranosides and α‐D‐galactosides decreased with increasing molecular weight. Seedling damage and growth retardation were paralleled by galactose accumulation in the substrate. Rupture of tonoplast and evagination of the nuclear envelope suggests that this sugar may alter some factor(s) responsible for the maintenance of membrane permeability.