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ASSESSMENT OF EVOLUTIONARY AFFINITIES IN GOSSYPIUM BY PROTEIN ELECTROPHORESIS
Author(s) -
Johnson B. Lennart,
Thein Mating Myint
Publication year - 1970
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1970.tb09912.x
Subject(s) - biology , affinities , genome , evolutionary biology , linum , genomic dna , electrophoresis , botany , gel electrophoresis of proteins , gel electrophoresis , polyacrylamide gel electrophoresis , genetics , dna , gene , biochemistry , enzyme
Protein band patterns from 25 species of Gossypium were obtained by electrophoresis of crude seed extracts on polyacrylamide gel. Band homologies between species were verified by electrophoresis of a mixture of their extracts. The patterns were found to be largely consistent with the conventional classification of the diploids into 6 genomic groups, A–F. However, G. triphyllum and G. bickii showed unique patterns differing respectively from those of the B and C groups, and G. australe showed closer affinity with the Arabian E‐ than with the Australian C‐genome species. Affinities among the D‐genome species were different from those implied by their former grouping into taxonomic sections but remarkably similar to those indicated in the most recent taxonomic revision of the genus. They were classifiable into two subgroups, β and ɛ. The clustering pattern of the diploids based on correlation coefficients calculated from densitometer curves of the electrophoretic spectra suggested that the genomic groups were derived from an African progenitor type, and that the American β and ɛ subgroups, most closely related to the African B‐ and the Arabian E‐genome groups respectively, evolved under comparative mutual isolation, possibly separated by the Tertiary Amazonas basin.