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THE KINETICS OF INITIAL NUCLEAR EXCHANGE IN COMPATIBLE AND NONCOMPATIBLE MATINGS OF SCHIZOPHYLLUM COMMUNE
Author(s) -
Leary John V.,
Ellingboe Albert H.
Publication year - 1970
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1970.tb09787.x
Subject(s) - biology , mating type , dikaryon , mycelium , mating , kinetics , botany , genetics , gene , physics , quantum mechanics
The effect of mating‐type factors on exchange of nuclei between mycelial fragments mated in liquid culture was studied. Evidence for exchange of nuclei was based on three criteria for detecting nuclei from both mating partners in mycelial fragments of the developing dikaryon or hetero‐karyon. The kinetics of nuclear exchange were shown to be relatively independent of the method used. It was shown that the kinetics of nuclear exchange in the first 96 hr are different in the compatible (A41 B41 × A42 B42) , common‐A ( A42 B42 × A42 B41) , common‐B (A41 B42 × A42 B42) , and common‐ AB ( A41 B41 × A41 B41) matings. In all four types of matings, the percentage of fragments possessing both types of nuclei 12‐24 hr after mating is nearly equal. After this time, significant differences appear in the patterns for compatible vs. the three non‐compatible matings and the common‐ A vs. the common‐ B and common‐ AB matings. The percentage of mycelial fragments possessing both types of nuclei throughout the 96‐hr test period is similar for both the common‐ B and common‐ AB matings. The kinetics of nuclear exchange were shown to be independent of the particular mating‐type alleles and nutritional markers used. When the efficiency of nuclear exchange in complete and minimal media was compared, it was shown that nuclear exchange occurred more rapidly and synchronously in minimal medium. This difference is not due to growth differences in the two media. These data indicate that the earliest mating interactions, i.e., hyphal anastomosis and nuclear exchange, are independent of the mating‐type factors but that subsequent events are determined by these genes.