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TEMPORAL AND SPATIAL CHANGES IN PEROXIDASE ACTIVITY DURING FRUIT DEVELOPMENT IN ENCYCLIA TAMPENSIS (ORCHIDACEAE)
Author(s) -
Alvarez Marvin R.
Publication year - 1968
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1968.tb07419.x
Subject(s) - ovule , peroxidase , biology , orchidaceae , botany , pollination , megaspore , horticulture , enzyme , biochemistry , pollen
Peroxidase activity in the fruit of Encyclia tampensis (Lindl.) Small was investigated at various stages of growth and correlated with the histological changes of megasporogenesis and ovule development. Sites of peroxidase activity in the developing fruit were demonstrated histochemically and soluble proteins were resolved electrophoretically at progressive stages of growth. Peroxidase activity increases linearly with fruit diameter and wet weight and is highest in the portion of the fruit containing the developing ovules. While activity is initially confined to the ovulary walls, the ovules show a marked increase in peroxidase activity which is correlated with the lignification of the integuments in the later phases of development. The peroxidase activity initiated by pollination appears entirely to be that of a single isozymic form.