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NUTRITION OF THE GREEN ALGA GOLENKINIA
Author(s) -
Ellis Richard J.,
Machlis Leonard
Publication year - 1968
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1968.tb07416.x
Subject(s) - biology , thiamine , strain (injury) , nitrate , ammonium , urea , autotroph , mutant , algae , ammonia , nitrogen , botany , biochemistry , food science , bacteria , chemistry , ecology , organic chemistry , anatomy , genetics , gene
Defined media were established for four strains of Golenkinia . Strains 929 and 930 require thiamine and vitamin B 12 , strain 931 requires the latter and is stimulated by thiamine; only strain 320 and a mutant of strain 931 are capable of completely autotrophic growth. The optimal nitrogen concentration is 0.025 m except for strain 931 where it is 0.015 m and the requirement may be met by nitrate, ammonium, or urea. Optimal levels of the other components are: 0.0025 m KH 2 PO 4 , 0.0005 to 0.001 m MgSO 4 , and 50 ml of a stock trace‐element solution per liter. Only strain 931 required calcium; however, its addition stimulates the growth of strains 929 and 930. The optimal p H before autoclaving is 6.8. Maximal growth rates of two to three cell doublings per day and yields of 26 to 30 (log 2 ) cells per ml were obtained. These growth data compare favorably with those for other unicellular green algae.