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THE ROOTING OF LIQUID‐GROWN ASPEN CALLUS
Author(s) -
Winton Lawson L.
Publication year - 1968
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1968.tb06956.x
Subject(s) - callus , biology , kinetin , tissue culture , explant culture , botany , liquid medium , agar , agar plate , horticulture , chromatography , chemistry , biochemistry , in vitro , genetics , bacteria
Liquid‐grown callus was used to study the nutritional requirements for rooting of a triploid form of normally diploid quaking aspen ( Populus tremuloides Michx.). In modified Wolter's liquid medium, tan rough‐surfaced spheres of callus grew rapidly when supplied with a high concentration of 2,4‐D (0.5 mg/liter), but light‐yellow uniformly smooth and firm spheres grew more slowly with a low level of 2,4‐D (0.04 mg/liter) plus kinetin. When tissue was grown uncut in liquid for 1, 2, or 3 months, then subcultured to a low‐2,4‐D agar medium, rooting increased primarily with the age of the source tissue rather than the initial explant size. The surface of the youngest tissue source was almost smooth, but free columnar proliferations extended out from the surface for two or three cells in 2‐month‐old tissue and for three to six cells in the 3‐month‐old tissue. The relationship of increased rooting with increased surface cell‐proliferation of older tissue was not determined anatomically.