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PRELIMINARY INVESTIGATION OF THE DOUBLE‐DIFFUSION TECHNIQUE AS A TOOL IN DETERMINING RELATIONSHIPS AMONG SOME MYXOMYCETES, ORDER PHYSARALES
Author(s) -
Franke Robert G.
Publication year - 1967
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1967.tb10753.x
Subject(s) - physarum polycephalum , physarum , biology , slime mold , plasmodium (life cycle) , mycetozoa , botany , taxonomy (biology) , microbiology and biotechnology , biochemistry , dictyostelium discoideum , parasite hosting , world wide web , computer science , gene
Double‐diffusion technique was used to investigate myxomycete relationships within the order Physarales. Extracts of plasmodia of 22 slime mold isolates were reacted with five antisera produced to Plasmodia of Didymium nigripes, Physarella oblonga, Physarum polycephalum, Physarum gyrosum and Fuligo septica. Two isolates of Fuligo septica tested alike. Four isolates of Physarum pusillum did not test alike, and no valid conclusion of the relationship of this species was possible. These isolates showed strong serological affinity: (1) Physarum gyrosum, Physarella oblonga , two isolates of Fuligo septica , and possibly two isolates of Physarum pusillum , and Physarum tenerum ; (2) Physarum polycephalum and Physarum flavicomum ; (3) Fuligo septica and many of the species tested; (4) Didymium nigripes and at least one isolate of Didymium iridis. In most cases serologial relationships among species tested did not coincide with current taxonomy based on morphology of fructification.