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AUXIN‐CYTOKININ CONTROL OF SECONDARY VASCULAR TISSUE FORMATION IN ISOLATED ROOTS OF RAPHANUS
Author(s) -
Torrey John G.,
Loomis Robert S.
Publication year - 1967
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1967.tb10738.x
Subject(s) - auxin , cytokinin , raphanus , biology , cambium , cyclitol , vascular cambium , vascular tissue , botany , sucrose , biochemistry , inositol , xylem , receptor , gene
A comparative study was made of the effectiveness of various hormone and metabolite mixtures in inducing vascular cambium initiation and secondary vascular tissue formation in isolated first‐transfer roots of the radish, Raphanus sativus L. ‘White Icicle,‘ when provided to the cut basal end of the root grown in sterile culture. An auxin, such as indoleacetic acid (IAA) at 10 –5 m , a cytokinin, such as 6‐benzylamino purine at 5 × 10 –6 m , a cyclitol, such as myo ‐inositol at 5 × 10 –4 M and sucrose at 8% were all required for maximum response. Requirements for auxin and cytokinin were absolute; in their absence no cambium was formed. The addition of cyclitol, while not an absolute requirement for cambium initiation, increased the magnitude of the response markedly. Alternative auxins such as α ‐naphthaleneaeetic acid and 2,4‐dichlorophenoxyacetic acid were equally as effective as IAA. Alternative effective cytokinins included 6‐furfurylaminopurine, 6‐phenylaminopurine and 6‐(γ,γ‐dimethylallylamino)purine. Alternative cyclitols equivalent to myo ‐inositol were seyllitol and pinitol. Other related cyclitols tested were much less effective or totally inactive.