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ANALYSIS OF GENOME AND SPECIES RELATIONSHIPS IN THE POLYPLOID WHEATS BY PROTEIN ELECTROPHORESIS
Author(s) -
Johnson B. Lennart,
Barnhart David,
Hall Ove
Publication year - 1967
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1967.tb10737.x
Subject(s) - biology , subspecies , polyploid , genome , homologous chromosome , genetics , albumin , botany , polyacrylamide gel electrophoresis , gene , zoology , biochemistry , enzyme
Protein spectra from 4 n and 6 n species of Triticum were obtained by electrophoresis of seed extracts on polyacrylamide gels. Homologies among the species with regard to protein bands were tested by comparing each species with the standard T. dicoccum in a protein mixture spectrum. By reference to two pairs of homologous bands, each spectrum was then adjusted to the migration velocity of the standard by photographic enlargement. The homologies were found to be consistent with evidence from conventional methods regarding genome relationships among the Triticum polyploids. T. dicoccoides and other known AABB tetraploids showed nine fast‐moving albumin homologues, while T. timopheevi and other known AAGG tetraploids showed seven. The two genomic groups had five albumin bands in common. The hexaploid (AABBDD) subspecies showed 12 albumin homologues, 9 of which were also homologous with the 9 of the AABB tetraploids and 3 of which were attributed to the D‐genome donor. Differences among species within each of the tetraploid genomic groups and among the hexaploid subspecies were largely confined to the slow‐moving bands of the gliadin series.