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QUINONE DISTRIBUTION IN HORSE‐CHESTNUT CHLOROPLASTS, GLOBULES AND LAMELLAE
Author(s) -
Barr Rita,
Magree Lynette,
Crane F. L.
Publication year - 1967
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1967.tb06931.x
Subject(s) - chloroplast , biology , sonication , chlorophyll , chlorophyll a , plastoquinone , botany , hill reaction , fraction (chemistry) , chromatography , biochemistry , chemistry , thylakoid , gene
The quinone content of whole and sonicated horse‐chestnut chloroplasts was studied over a period of 6 months. Whole chloroplasts show a steady increase of plastoquinone A and C concentrations from May to September. By September 1 about 0.5 μmoles PQ A and about 0.3 μmoles PQ C plus D per mg chlorophyll are found in whole chloroplasts. The osmiophilic globule fraction of sonicated chloroplasts contains traces of PQ A but no PQ C in May. By October 5 equal amounts of PQ A and PQ C plus D (0.15 μmoles per mg chlorophyll) are found in horse‐chestnut globules. By Oct. 15 the PQ A content increases at least 20‐fold, the PQ C content at least 7‐fold. The lamellae fraction of sonicated horse‐chestnut chloroplasts contains 0.05 μmoles PQ A and 0.03 μmoles PQ C plus PQ D in May. By October 15 about 0.3 μmoles PQ A and 0 1 μmoles PQ C per mg chlorophyll can be found in lamellae. The total amount of plastoquinones accumulated in the globules accounts for up to 20% of the total accumulation in the chloroplast during the season.