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INHIBITION OF TWO‐DIMENSIONAL GROWTH AND SUPPRESSION OF RIBONUCLEIC ACID AND PROTEIN SYNTHESIS IN THE GAMETOPHYTES OF THE FERN, ASPLENIUM NIDUS, BY CHLORAMPHENICOL, PUROMYCIN AND ACTINOMYCIN D
Author(s) -
Raghavan V.,
Tung Heng Fong
Publication year - 1967
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1967.tb06909.x
Subject(s) - puromycin , chloramphenicol , biology , fern , protein biosynthesis , dactinomycin , gametophyte , rna , uridine , growth inhibition , biochemistry , antibiotics , cell growth , botany , cycloheximide , pollen , gene
Chloramphenicol and puromycin at appropriate concentrations inhibited the induction of two‐dimensional growth in the gametophytes of the fern Asplenium nidus without drastically inhibiting germination and continued filamentous growth. Similar responses to actinomycin D were reported earlier. Radioautographic techniques were employed to study the pattern of ribonucleic acid and protein synthesis in gametophytes which were treated with chloramphenicol, puromycin and actinomycin D. Uptake of H 3 ‐uridine into ribonucleic acid was strongly inhibited by all three antibiotics. Chloramphenicol and puromycin were not as effective as actinomycin D in inhibiting H 3 ‐leucine incorporation. The results are discussed in relation to the quality of light and antibiotics on two‐dimensional growth in the gametophytes.