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NUTRITIONAL REQUIREMENTS OF FRAXINUS CALLUS CULTURES
Author(s) -
Wolter Karl E.,
Skoog Folke
Publication year - 1966
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1966.tb07333.x
Subject(s) - kinetin , callus , liter , biology , sucrose , gibberellic acid , auxin , botany , chemically defined medium , pyridoxine , agar , biochemistry , horticulture , tissue culture , germination , genetics , gene , bacteria , in vitro , endocrinology
A satisfactory synthetic medium has been developed for continuous growth of Fraxinus pennsylvanica Marsh. callus cultures. The medium contains modified Reinert and White (1956) inorganic nutrient solution with 5 mg/liter Fe as NaFe‐EDTA and supplemented with myoinositol 10 mg/liter, pyridoxine HCl 0.1 mg/liter, 2,4‐dichlorophenoxyacetic acid 0.04 mg/liter, kinetin 1 mg/liter, sucrose 20 g/liter, and agar 10 g/liter. myo ‐Inositol, pyridoxine and an auxin are essential. α‐Naphthaleneacetic acid is an effective alternate auxin. Kinetin and to some extent gibberellic acid improve the yields. Thiamine has no effect.