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INDUCTION OF APOGAMY IN MEGAGAMETOPHYTES OF ZAMIA INTEGRIFOLIA
Author(s) -
Norstog Knut
Publication year - 1965
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1965.tb07276.x
Subject(s) - biology , ploidy , ovule , botany , asparagine , kinetin , auxin , embryo , datura , tissue culture , biochemistry , genetics , pollen , amino acid , gene , in vitro
Ovules of Zamia integrifolia Ait. were dissected and megagametophytes and embryos excised and cultured. Induction of apogamous, haploid roots and leaves by auxin and kinetin, in combination with glutamine, asparagine and alanine, was observed. The organs resembled their normal diploid counterparts. 2,4‐D at 1 ppm was more effective than 1 ppm IAA, but less effective than 5 ppm IAA. The highest percentage of apogamy was 59% after 5 months of culture. In several cultures embryoids were formed both by diploid excised embryos and by haploid megagametophtyes.