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ACRYLAMIDE GEL ELECTROPHORESIS OF SOLUBLE PLANT PROTEINS: A STUDY ON PEA SEEDLINGS IN RELATION TO DEVELOPMENT
Author(s) -
Steward F. C.,
Lyndon R. F.,
Barber J. T.
Publication year - 1965
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1965.tb06770.x
Subject(s) - sativum , biology , pisum , seedling , polyacrylamide gel electrophoresis , electrophoresis , acrylamide , gel electrophoresis , two dimensional gel electrophoresis , botany , neurospora , proteome , pea protein , biochemistry , chromatography , gene , proteomics , enzyme , chemistry , neurospora crassa , organic chemistry , mutant , copolymer , polymer
The application of the technique of acrylamide gel electrophoresis to the separation of components of the soluble proteins of plants is described. The reproducibility of the results is demonstrated with reference to Neurospora proteins, and suitable procedures to document the results are described. These techniques have been applied to the soluble protein complement of segments along the axis of the root of Pisum sativum and of other parts of the seedling. The observations are interpreted in the light of current views on protein synthesis and cellular development.