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ESTIMATION OF NUCLEAR RATIOS DIRECTLY FROM HETEROKARYOTIC MYCELIA IN SCHIZOPHYLLUM
Author(s) -
Snider Philip J.
Publication year - 1963
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.1002/j.1537-2197.1963.tb12232.x
Subject(s) - heterokaryon , mycelium , spore , biology , schizophyllum commune , biological system , botany , genetics , gene , mutant
Snider, P. J. (U. California, Berkeley.) Estimation of nuclear ratios directly from heterokaryotic mycelia in Sehizophyllum. Amer. Jour. Bot. 50(3): 255–262. Illus. 1963.—The principles used to analyze nuclear ratios with macerated mycelium are similar in part to those of classic methods utilizing spore suspensions, but some distinctive features are evident in the present method. It is simple, adapted particularly for analyzing highly disparate ratios, and probably suitable for the simultaneous comparison of ratios in mycelium and spores. The technique can be combined with others, as described, to devise a test suggesting whether random or non‐random spatial distributions of nuclei exist in heterokaryons with disparate ratios. Direct analysis, as described and evaluated here, was used in a study reported in a companion paper, and numerous applications in fungi seem probable.